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The sex of an unborn fetus can be determined by observation of interphase cells (see amniotic centesis and Barr body).Six different characteristics of karyotypes are usually observed and compared: The normal human karyotypes contain 22 pairs of autosomal chromosomes and one pair of sex chromosomes (allosomes).Lev Delaunay is applied after cells have been arrested during cell division by a solution of colchicine usually in metaphase or prometaphase when most condensed.In order for the Giemsa stain to adhere correctly, all chromosomal proteins must be digested and removed.It is New Latin from Ancient Greek κάρυον karyon, "kernel", "seed", or "nucleus", and τύπος typos, "general form").The next stage took place after the development of genetics in the early 20th century, when it was appreciated that chromosomes (that can be observed by karyotype) were the carrier of genes.But when they obtained a couple more specimens they confirmed [their findings].

When they looked at the karyotype of the closely related Indian muntjac, Muntiacus muntjak, they were astonished to find it had female = 6, male = 7 chromosomes. They kept quiet for two or three years because they thought something was wrong with their tissue culture ...Attention is paid to their length, the position of the centromeres, banding pattern, any differences between the sex chromosomes, and any other physical characteristics.The study of whole sets of chromosomes is sometimes known as karyology.The low record is held by the nematode Parascaris univalens, where the haploid n = 1; and an ant: Myrmecia pilosula.The existence of supernumerary or B chromosomes means that chromosome number can vary even within one interbreeding population; and aneuploids are another example, though in this case they would not be regarded as normal members of the population.